Yeast mitochondria contain ATP-sensitive, reversible actin-binding activity.
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چکیده
منابع مشابه
Liver mitochondria contain an ATP-dependent, vanadate-sensitive pathway for the degradation of proteins.
A large fraction (30-50%) of the various proteins synthesized within isolated rat liver mitochondria were degraded to amino acids within 60 min after synthesis. Incomplete mitochondrial polypeptides resulting from the incorporation of puromycin were degraded even more extensively (80% per hr). Protein breakdown was measured by the appearance of acid-soluble radioactivity and by the disappearanc...
متن کاملATP-induced unspecific channel in yeast mitochondria.
ATP induced swelling of isolated yeast mitochondria suspended in an isoosmotic solution of potassium gluconate. Valinomycin stimulated the swelling rate, indicating that K+ influx in the presence of ATP is rate-controlling. This swelling was inhibited by ADP, phosphate (probably acting on the external face of the inner membrane), and Mg2+, which forms a complex with ATP. ATP-induced swelling di...
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Isolated kidney mitochondria swell when incubated in hyposmotic solutions containing K+ salts in a manner inhibited by ATP, ADP, 5-hydroxydecanoate, and glibenclamide and stimulated by GTP and diazoxide. These results suggest the existence of ATP-sensitive K+ channels in these mitochondria, similar to those previously described in heart, liver, and brain. Renal mitochondrial ATP-sensitive K+ up...
متن کاملCapping protein binding to actin in yeast
The mechanism by which capping protein (CP) binds barbed ends of actin filaments is not understood, and the physiological significance of CP binding to actin is not defined. The CP crystal structure suggests that the COOH-terminal regions of the CP alpha and beta subunits bind to the barbed end. Using purified recombinant mutant yeast CP, we tested this model. CP lacking both COOH-terminal regi...
متن کاملDynamics of Mitochondria and Actin Cytoskeleton Interactions in Budding Yeast
In the budding yeast, S. cerevisiae, mitochondrial distribution involves movements of the organelle from the mother cell to the bud (anterograde movement) and from the bud to the mother (retrograde movement). Both types of movements require actin cables, dynamic bundles of F-actin filaments, which undergo continuous retrograde flow from the bud into the mother and provide tracks for mitochondri...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1994
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.5.7.807